Universal Primer List
Universal primers are PCR/sequencing primers that bind to a sequence found in many plasmid cloning vectors, most of which are derived from pUC vectors (which in turn come from pBR322). These sequences were defined as good PCR and sequencing sites as they flank the multiple cloning site where an inserted DNA sequence would be put.
| No | Primer Name | Primer Sequence |
|---|---|---|
| 1 | M13 Reverse (-27) | 5'-GGA AAC AGC TAT GAC CAT G-3' |
| 2 | M13 Forward (-41) | 5'-GGT TTT CCC AGTC ACG AC-3' |
| 3 | M13 Forward (-20) | 5'-GTA AAA CGA CGG CCA GTG-3' |
| 4 | M13 Reverse (-48) | 5'-AGC GGA TAA CAA TTTC ACA C-3' |
| 5 | SP6 | 5'-TAC GAT TTA GGT GAC ACT ATA G-3' |
| 6 | T3 | 5'-CAA TTA ACC CTC ACT AAA GG-3' |
| 7 | T7 | 5'-TAA TAC GAC TCA CTA TAG GG-3' |
| 8 | T7 EEV | 5'-ATGTCGTAATAACCCCGCCCCG-3' |
| 9 | T7 Reverse | 5'-TAGTTATTGCTCAGCGGTGG-3' |
| 10 | T7 Term | 5'-GCT AGT TAT TGC TCA GCG G-3' |
| 11 | pBluescript KS | 5'-TCG AGG TCG ACG GTA TC-3' |
| 12 | pBluescript SK | 5'-CGC TCT AGA ACT AGT GGA TC-3' |
| 13 | 3'pGEX | 5'-CCG GGA GCT GCA TGT GTC AGA GG-3' |
| 14 | 5'pGEX | 5'-GGG CTG GCA AGC CAC GTT TGG TG-3' |
| 15 | GST-Tag | 5'-ACC CAA TGT GCC TGG ATG CG-3' |
| 16 | pTrcHis-Forward | 5'-GAG GTA TAT ATT AAT GTA TCG-3' |
| 17 | pTrcHis-Reverse | 5'-GAT TTA ATC TGT ATC AGG |
| 18 | CMV-Forward | 5'-CGC AAA TGG GCG GTA GGC GTG-3' |
| 19 | CMV-Reverse | 5'-AGT AGG AAA GTC CCG TAA GG-3' |
| 20 | EGFP-C | 5'-CAT GGT CCT GCT GGA GTT CGT G-3' |
| 21 | EGFP-N | 5'-CGT CGC CGT CCA GCT CGA CCA-3' |
| 22 | BGH-Reverse | 5'-TAG AAG GCA CAG TCG AGG-3' |
| 23 | pQEproseq | 5'-CCC GAA AAG TGC CAC CTG-3' |
| 24 | pQErevseq | 5'-GTT CTG AGG TCA TTA CTG G-3' |
| 25 | Intein Forward | 5'-CCC GCC GCT GCT TTT GCA CGT GAG-3' |
| 26 | 5'-pBabe-Seq | 5'-CTT TAT CCA GCC CTC AC-3' |
| 27 | 3'-pBabe-Seq | 5'-ACC CTA ACT GAC ACA CATT CC-3' |
| 28 | -96 glll Sequencing Primer | 5'-CCC TCA TAG TTA GCG TAA CG-3' |
| 29 | GAL1 Forward | 5'-AAT ATA CCT CTA TAC TTT AAC GTC-3' |
| 30 | pBAD Forward | 5'-ATG CCA TAG CAT TTT TAT CC-3' |
| 31 | pBAD Reverse | 5'-GAT TTA ATC TGT ATC AGG |
| 32 | pTRE 3' | 5'-CCA CAC CTC CCC CTG AAC-3' |
| 33 | pTRE 5' | 5'-CGC CTG GAG ACG CCA TCC-3' |
| 34 | pYESTrp Forward | 5'-GAT GTT AAC GAT ACC AGC C-3' |
| 35 | pYESTrp Reverse | 5'-GCG TGA ATG TAA GCG TGA C-3' |
| 36 | RVprimer3 | 5'-CTA GCA AAA TAG GCT GTC CC-3' |
| 37 | Rvprimer4 | 5'-GAC GAT AGT CAT GCC CCG CG-3' |
| 38 | GLprimer 1 | 5'-TGT ATC TTA TGG TAC TGT AAC TG-3' |
| 39 | GLprimer 2 | 5'-CTT TAT GTT TTT GGC GTC TTC CA-3' |
| 40 | SeqL-A (ATTL1) | 5'-GCG AGA GTA GGG AAC TGC-3' |
| 41 | SeqL-B (ATTL2) | 5'-AAC ATC AGA GAT TTT GAG ACA C-3' |
| 42 | SV40-pArev | 5'-CCT CTA CAA ATG TGG TAT GG-3' |
| 43 | SV40-Promoter | 5'-GCC CCT AAC TCC GCC CAT CC-3' |
| 44 | U6 Primer | 5'-GGG CAG GAA GAG GGC CTA T-3' |
| 45 | Xpress Forward | 5'-TAT GGC TAG CAT GAC TGG T-3' |
| 46 | EBV-Rev primer | 5'-GTG GTT TGT CCA AAC TCA TC-3' |
