Universal Primers

Submission Form

SeqScanner (PC)

               Discount for volume submissions               All samples should be accompanied by a completed submission form with PO# or credit card #

     Lone Star Labs, Inc. is a DNA sequencing company committed to providing our customers with the highest quality results and services in both industry and academia. Since DNA sequencing is our main focus, we can offer specialized attention to each and every sample we receive. Our lab is directed by PhDs with extensive molecular biology experience so that we can assure our customers of the latest technology and techniques.

Advantages:

• Fast turnaround time (in most cases 24-48 hrs).
• BigDye^TM  Terminator Version 3.1
• Competitive pricing (call for your special quote).
• All project sizes accepted.
• All results are available via email or web posting.
• All templates accepted--- including BAC& genomic 
• See our Helpful Hints for recommendations of templates.
• Free pickup & delivery for Houston and surrounding areas.

Recommendations:

For best results, we suggest the following; (Also check our helpful hints page!!!!! )

* Prepare the template DNA and primer in DI water...Do not use TE.
* Primer must be at least 18-24 bp with a melting point temperature of 50^oC higher.
* The primer must be at a concentration of 3.2-10 pmol/ul.
* Indicate type of DNA template being sequenced; ds, ss, or pcr DNA (include size)
* For each reaction: 50-100 ng ssDNA, 0.5-1.0 ug cosmid DNA
   0.5-1.0 ug dsDNA, 1-2 ug BAC,  2-3 ug bacterial genomic DNA

*For successful direct genomic sequencing we strongly recommend a high quality genomic prep.    

*For recommended DNA isolation methods please contact us for details at sequence@lslabs.com.  We recommend Mo Bio Laboratories for DNA isolation kits for the ease and quality of the preps as well as the low cost per sample. 

    Carefully check all templates for proper concentration and purity... LOW TEMPLATE CONCENTRATION, SALT,and or ALCOHOL contamination in preps will result in failure of sequencing reaction !!!   

* For sequencing a PCR product: 

   100-200 bp 1-3 ng DNA
   200-500 bp 3-10 ng DNA
   500-1000 bp 5-20 ng DNA
   1000-2000 bp 10-40 ng DNA
   >2000 bp 40-100 ng DNA
*Adjust to a final volume of 1-10ul and label tubes w/ DNA concentration and date.

Note:  It is the responsibility of our clients to check all electrophoregram for:

mis-calls

spacing

we do not edit any of the sequence

NBCI

PubMed

ABI